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smooth muscle nucleofection solution  (Lonza)


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    Lonza smooth muscle nucleofection solution
    Smooth Muscle Nucleofection Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smooth muscle nucleofection solution/product/Lonza
    Average 90 stars, based on 1 article reviews
    smooth muscle nucleofection solution - by Bioz Stars, 2026-05
    90/100 stars

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    <t>Nucleofection</t> with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.
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    primary smooth muscle cells nucleofection solution  (Amaxa)
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    <t>Nucleofection</t> with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.
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    <t>Nucleofection</t> with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.
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    Nucleofection with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.

    Journal: PLoS ONE

    Article Title: Upregulation of Intermediate-Conductance Ca 2+ -Activated K + Channels (KCNN4) in Porcine Coronary Smooth Muscle Requires NADPH Oxidase 5 (NOX5)

    doi: 10.1371/journal.pone.0105337

    Figure Lengend Snippet: Nucleofection with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.

    Article Snippet: 4 day serum-starved cultured CSMCs were trypsinized, placed in basic smooth muscle nucleofection solution (Amaxa), and transfected with pAP-1 Luc Cis-Reporter plasmid in which firefly luciferase gene expression in the reporter plasmid is controlled by a synthetic promoter containing direct repeats of the AP-1 transcription recognition sequence (1.0ug, Stratagene 219074).

    Techniques: Expressing, Control